Please email firstname.lastname@example.org for an up-to-date excel list with all included embryonic and fetal material, including information such as gestational age and reason for termination of pregnancy.
Age is estimated in the hospital before termination of pregnancy by the treating gynaecologist based upon the crown-rump-length on ultrasound.
Upon retrieval, the specimens are anonymised. All available pre- and postnatal clinical information such as reason for termination, ultrasound findings, karyotype, micro-array data and results of whole-exome sequencing, is systematically collected before anonymization. In addition, important parameters associated with termination of pregnancy, such as methodology and last registered positive heart action are documented.
If the fetus is dissected, samples of each organ system are collected and the tissue samples are stored using various methods to facilitate a variety of analyses. This includes storage in RNAlater, snapfrozen tissue, culture medium and any remaining tissue that is sufficiently intact for morphological or histological examination following organ isolation is fixed in 4% (w/v) paraformaldehyde (PFA) in phosphate buffered saline (PBS). A complete list of tissue types and storage methods can be found in our dissection protocol, please email email@example.com for more information.
We monitor the quality of the collected samples, by randomly sampling 1% of the total number of samples and extract RNA using the ReliaPrep™ RNA Tissue Miniprep System Kit (Promega, Wis, USA). Subsequently, RNA quality is assessed using a 4200 Agilent TapeStation System (Agilent, Ca, USA), which estimates the degree of RNA degradation reflected in a RIN on a scale of 0 – 10.
The image repository contains complete high resolution (3D) datasets of fetuses imaged using ultrasound, 3T MRI, 7T MRI, and/or micro-CT.
Ethical permission for the collection of the material for research has been obtained at the Amsterdam UMC, Amsterdam (METC 2016_285, #B2017369). Local ethical approval for applicants based in the Netherlands is usually not necessary if the proposed project lies within the activities for which the Dutch Fetal Biobank has existing ethical approval (primarily molecular studies and imaging studies).
Applicants based in other countries should be aware that it is their responsibility to make sure that local ethical approval is arranged and that the use of human embryonic and fetal material complies with their own country’s national ethical practices and guidelines. The Dutch Fetal Biobank cannot be held responsible for users’ actions.
If the Dutch Fetal Biobank is sending embryonic and/or fetal materials then an MTA will be drafted, in the case of imaging data this should be a DTA. Information about the procedure can be obtained through firstname.lastname@example.org.
We strongly encourage publications of research using material from the Dutch Fetal Biobank. All publications arising from the use of material provided by the Dutch Fetal Biobank must acknowledge the contribution of the Dutch Fetal Biobank within the manuscript. Please follow our Ethics & Citation policy.